Composite

Part:BBa_K3763024:Design

Designed by: Guokai Wang   Group: iGEM21_WHU-China   (2021-10-18)


the expression operator in pET26b+ and CDS of PctA, with OmpA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 326
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 310


Design Notes

The reason that we chose His-tag is that small 6xHis tail might not change the ability of our interested proteins so we can not only purify the interested proteins but also directly test its inhibition ability against P. acne.


Source

The T7 promotor,lac operator,RBS 1,6*His tag and T7 terminator come from the plasmid pET26b+, PctA CDS comes from basic part BBa_3763022 and OmpA leader peptide comes from BBa_K387007


References